Journal: Transfusion

Article Title: The feasibility and efficacy of subcutaneous plerixafor for mobilization of peripheral blood stem cells in allogeneic HLA–identical sibling donors: results of the HOVON‐107 study

doi: 10.1111/trf.15037

Figure Lengend Snippet: Composition of the grafts obtained from healthy adult sibling donors

Article Snippet: T lymphocytes were purified from peripheral blood using a human whole blood CD3 positive selection kit (STEMCELL Technologies, Inc.) according to the manufacturer's instructions.

Techniques:

Journal: Transfusion

Article Title: The feasibility and efficacy of subcutaneous plerixafor for mobilization of peripheral blood stem cells in allogeneic HLA–identical sibling donors: results of the HOVON‐107 study

doi: 10.1111/trf.15037

Figure Lengend Snippet: Chimerism in peripheral blood (PB), bone marrow (BM), and CD3 selected cells at 3, 6, and 12 months after transplant

Article Snippet: T lymphocytes were purified from peripheral blood using a human whole blood CD3 positive selection kit (STEMCELL Technologies, Inc.) according to the manufacturer's instructions.

Techniques:

Journal: Transfusion

Article Title: The feasibility and efficacy of subcutaneous plerixafor for mobilization of peripheral blood stem cells in allogeneic HLA–identical sibling donors: results of the HOVON‐107 study

doi: 10.1111/trf.15037

Figure Lengend Snippet: Immunologic reconstitution. Numbers of CD3+; CD3+/CD4+; CD3 + CD8+; CD19+, and NK cells in the peripheral blood at 3, 6, 12, and 24 months after transplant. At 12 months: CD3 + cells: median, 0.84 × 10 9 /L (0.33–1.65); normal range, 0.66–2.10. CD3+/CD4+ cells: median, 0.30 × 10 9 /L (0.17–0.75); normal range, 0. 32–1.36. CD3+/CD8+ cells: median, 0.45 × 10 9 /L (0.11–1.24); normal range, 0.15–0.88. CD3–/CD16+/CD56+ NK cells: median, 0.20 × 10 9 /L (0.13–0.52); normal range, 0.09–0.62. CD19+ cells: median, 0.27 × 10 9 /L (0.09–0.95); normal range, 0.10–0.5. Each box is drawn from the 25th percentile to the 75th percentile of the cell counts, and the horizontal bar within the box indicates the median cell count. The line up from the top of the box goes to the upper adjacent value as defined. The dots indicate cell counts larger than the upper adjacent value. [Color figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: T lymphocytes were purified from peripheral blood using a human whole blood CD3 positive selection kit (STEMCELL Technologies, Inc.) according to the manufacturer's instructions.

Techniques: Cell Counting

Journal: Cell reports

Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy

doi: 10.1016/j.celrep.2020.108434

Figure Lengend Snippet: (A) Baseline number of foci per nucleus as a function of age in CD3 + lymphocytes extracted from finger prick collection in 339 healthy donors (***p < 0.0001, deviation from zero of the slope of linear regression). Four donors in this cohort were considered healthy but were in remission from cancer at the time of the finger prick (shown as red dots). See also . (B) Distribution of spontaneous foci per nucleus after bead-based extraction of CD3 + lymphocytes from finger prick samples (finger prick), Ficoll-based extraction of PBMCs from buffy coat samples (PBMC baseline), and a further freeze-thaw cycle and plating in the cell culture media (PBMC 0 Gy). ***p < 0.0001, one-way ANOVA, Tukey’s post hoc test for multiple comparisons among all three groups: 1–2, 2–3, and 1–3. Boxplots show median ± quartiles ± min/max values.

Article Snippet: EasySep CD3+ Whole Blood Selection Kit , StemCell Technologies , Cat# 18081.

Techniques: Extraction, Cell Culture

Journal: Cell reports

Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy

doi: 10.1016/j.celrep.2020.108434

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: EasySep CD3+ Whole Blood Selection Kit , StemCell Technologies , Cat# 18081.

Techniques: Recombinant, Red Blood Cell Lysis, Selection, Flow Cytometry, Multiplex Assay, Software